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KMID : 0895820230330020001
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Journal of Oriental Rehabilitation Medicine
2023 Volume.33 No. 2 p.1 ~ p.18
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The Effects of Samul-tang-ga-dansam for Wound Healing
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Lee Eun-Byeol
Kim Hyeon-Ji
Kim Chae-Young
Choi Ji-Su
Woo Chang-Hoon
Kim Young-Jun
An Hee-Du
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Abstract
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Objectives The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samul-tang-ga-dansam water extract (SD) in woundinduced mice.
Methods The mice were divided into five groups (n=7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group.
The normal group had no wounds and the other groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively.
Antioxidant and anti-inflammatory protein levels were evaluated using western blot analysis. Skin tissue was analyzed by H&E, Masson’s trichrome staining method.
Results Oral administration of the SD significantly reduced the visible skin damage and decreased the reactive oxygen species and ONOO? activity of the serum. It significantly increased heme oxygenase-1, superoxide dismutase, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor α, interleukin (IL)-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22phox and p47phox and increased α-smooth muscle actin and COL1A1 protein expression in fibroblasts involved in connective tissue repair. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing.
Conclusions It is suggested that Samul-tang-ga-dansam has antioxidant and antiinflammatory effects and promotes wound tissue repair.
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KEYWORD
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Samultang-ga-dansam, Wound healing, Antioxidants
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